Janina DemelerSpeaker Biography

Janina Demeler

  • Studied Veterinary Medicine in Hannover
  • Moved to Australia and worked as a vet for a while, predominantly with large animals (cattle, sheep, horse)
  • Started PhD at University of Sydney under joined supervision of Nick Sangster and Georg von Samson-Himmelstjerna, graduated end of 2005
  • First Postdoc position in Hannover 2006 in PARASOL EU project
  • Degree of Specialist in Vet Parasitology 2008
  • Second PhD on molecular Parasitology at the University of Veterinary Medicine Hannover in 2009
  • Moved to Berlin end of 2009
  • Associate Professor since 11.2010
  • Main areas of research are diagnosis of endoparasites, epidemiology, and mechanisms of anthelmintic resistance in nematodes, in vitro assay development, tick-transmitted pathogens.

Abstract Submission

Novel diagnostic methods ? technical developments and their implementation in ruminant routine diagnostics and monitoring programmes

For the effective management and control of helminths in ruminants, three key questions are usually addressed: which parasite species are present, which one is mainly responsible for disease and how resistant is the present nematode population. In recent years, the rapid increase in technological evolution enabled development and implementation of new methods in this field of parasitology. However, despite the existence of a range of new methods it has been shown that the transfer into routine diagnostics and field monitoring is rather low. New technical developments use three main columns which are either DNA-based, including real time amplification and/or next generation sequencing, involve multiplexing of assays or address the development of pen-side diagnostic techniques. A range of new methods has been reported for their use in scientific research. For the identification of the species present in faecal samples a direct faecal PCR has been reported for a range of hosts which is compatible to detection methods such as high resolution melt, pyrosequencing or RFLP. A multiplex-tandem real time PCR was described to sufficiently enable identification as well as quantification of nematode species present in faeces of sheep using the Easy plex system. Deep sequencing has been investigated regarding the potential to diagnose resistance within a population of nematodes. For the detection of benzimidazole (BZ) resistance individual pyrosequencing assays are now available for 5 different nematode species and have been evaluated in BZ-resistance field surveys (sheep & cattle) in European countries. The Luminex xMAP technology was employed to develop a multiplex immunological bead-based assay for the simultaneous detection of pasture borne nematode infections in bovine milk and serum samples. A step towards pen-side application was the design of a LAMP assay for the rapid and highly sensitive detection of Haemonchus contortus in faecal samples of sheep. Despite this range of new techniques, robust, reliable, rapid and low-priced methods to be implemented in routine diagnostics are still missing. This accounts for the assessment of individual and herd health status as well as for the identification and quantification of the parasite species responsible for disease. One major constrain is the acceptance of farmers to spend money on diagnostic, particularly if a direct benefit in terms of increased productivity is not is not obvious. This talk aims at highlighting recent development in diagnostic techniques as well as their potential for implementation in routine testing.

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